Coding

Part:BBa_K902014:Design

Designed by: Himika Dastidar   Group: iGEM12_Calgary   (2012-09-28)

LacI(R0010)+mgtA riboswitch+GFP-LVA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1120


Design Notes

none

Source

mgtA riboswitch- synthesized

GFP-LVA- parts registry

LacI(R0010) -parts registry

Parts were constructed using basic biobrick construction.

References

Michael J. Cromie and Eduardo A. Groisman. Promoter and Riboswitch Control of the Mg2+ Transporter MgtA from Salmonella enterica. J Bacteriol. 2010 January; 192(2): 604–607.